CS23D2.0 is a web server for rapidly generating accurate 3D protein structures using
only assigned NMR chemical shifts as input. Unlike conventional NMR methods, which require NOE and/or J-coupling data, CS23D2.0 uses only chemical shift
information to generate a 3D structure of the protein of interest. CS23D2.0 accepts chemical shift files in either SHIFTY or BMRB formats and produces a set
of PDB coordinates for the protein in about 10-15 minutes. CS23D2.0 uses a combination of maximal subfragment assembly, chemical shift threading,
shift-based torsion angle prediction and chemical shift refinement to generate and refine the protein coordinates. Tests indicate that CS23D2.0 converges
(i.e. finds a solution) for about 90% of protein queries. The performance is dependent on the completeness of the chemical shift assignments and the similarity
of the query protein to known 3D folds. If you use the results of the CS23D2.0 server in a publication, please cite the following
Wishart DS, Arndt D, Berjanskii M, Tang P, Zhou J, Lin G.
CS23D: a web server for rapid protein structure generation using NMR chemical shifts and
sequence data. Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W496-502.